February 10, 2018

Download PDF by Kevan M. A. Gartland, Michael R. Davey: Agrobacterium Protocols

By Kevan M. A. Gartland, Michael R. Davey

Agrobacterium Protocols deals starting and skilled researchers the main accomplished number of step by step protocols for the genetic manipulation of crops utilizing Agrobacterium. the subjects variety from the upkeep of bacterial tradition collections to points of the metabolism and body structure of reworked tissues and transgenic vegetation. Drawing at the paintings of best scientists from laboratories around the globe, Agrobacterium Protocols offers a wealth of ideas for introducing particular DNA sequences into goal plant species and discusses the environmental implications of genetically engineered vegetation. Its particular techniques will facilitate speedy move of complicated suggestions to different laboratories and their exploitation in primary and utilized plant biology.

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1987) GUS fusion: p-glucuronidase as a sensltlve and versatile gene fusion marker m higher plants. EMBO J. 6,3901-3907. 21. , and Frommer, W. B. (1992) Non-destructive assay systems for detection of P-glucuronidase activity in higher plants Plant Mol Biol. Rep. 10,37-46. 22. , and Goldberg, R. B. (1990) Introduction of male sterility m plants by a chimerlc rlbonuclease gene Nature 347,737-74 1. 23 Green, P. , and Inouye, M. (1986) The role of antisense RNA m gene regulation. Annu Rev. Blochem.

5, 1755-1760. 26. Fobert, P. , Miki, B. L , and Iyer, V. N. (1991) Detection of gene regulatory signals m plants revealed by T-DNA-mediated fusions. Plant Mol. Biol 17,837-852. 27 Gierl, A. and Saedler, H. (1992) Plant-transposable elements and gene taggmg. Plant Mol. Biol. 19,39-49. , Redri, G. P , and Schell, J (1992) T-DNA insertional mutagenesis in Arabidopsis. Plant Mol. Biol 20,963-976. 29. Feldmann, K. A (1991) T-DNA insertlon mutagenesis in Arabidopsis: mutational spectrum. Plant J. 1,7 1-82 30.

And Schell, J (1983) Size, location and polarity of T-DNA-encoded transcripts m 58 An nopaline crown gall tumors; common transcripts in octopine and nopaline tumors. Cell 32,1045-1056. 14. , Costa, M. -B. (1990) Nopalme synthase promoter is wound inducible and auxin inducible. Plant Cell 2,225-233. 15. Benfey, P N. -H (1990) The cauliflower mosaic virus promoter: combinational regulation of transcription in plants. Science 250,959-966. 16. , and Pellow, J. W. (1990) A mutant neomycin phosphotransferase II gene reduces the resistance of transformants to antlblotic selection pressure.

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Agrobacterium Protocols by Kevan M. A. Gartland, Michael R. Davey


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